Journal article
Multi-Omic Analysis to Characterize Metabolic Adaptation of the E. coli Lipidome in Response to Environmental Stress
T Kralj, M Nuske, V Hofferek, MA Sani, TH Lee, F Separovic, MI Aguilar, GE Reid
Metabolites | Published : 2022
Abstract
As an adaptive survival response to exogenous stress, bacteria undergo dynamic remod-elling of their lipid metabolism pathways to alter the composition of their cellular membranes. Here, using Escherichia coli as a well characterised model system, we report the development and application of a ‘multi-omics’ strategy for comprehensive quantitative analysis of the temporal changes in the lipidome and proteome profiles that occur under exponential growth phase versus stationary growth phase conditions i.e., nutrient depletion stress. Lipidome analysis performed using ‘shot-gun’ direct infusion-based ultra-high resolution accurate mass spectrometry revealed a quantitative decrease in total lipid..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
FundingThis research was funded by the National Health and Medical Research Council, grant number APP1142750 (G.E.R., F.S., and M.-I.A.), and by the Australian Research Council, grant number DP190102464 (G.E.R). The APC was funded from DP190102464. The authors acknowledge support from Dr. Romain Huguet (Thermo Fisher Scientific, San Jose, CA, USA) for providing the FAIMS Pro source and the 213 nm UV laser used in this study as part of a collaborative research project with G.E.R.